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                                            补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法)

                                            ELISA Kit for Complement Component 3a (C3a)

                                            • 补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
                                            • 补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
                                            • 补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法) 实验结果图
                                            • SEA387Ra.jpg 标准曲线图
                                            • Certificate 通过ISO 9001、ISO 13485质量体系认证

                                            特异性

                                            本试剂盒用于检测补体成分3a(C3a),经检测与其它相似物质无明显交叉反应。
                                            由于受到?#38469;?#21450;样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

                                            回收率

                                            分别于定值血清及血浆样本中加入一定量的补体成分3a(C3a)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

                                            样本 回收率范围(%) 平均回收率(%)
                                            serum(n=5) 78-95 89
                                            EDTA plasma(n=5) 82-104 92
                                            heparin plasma(n=5) 84-102 94

                                            精密度

                                            精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
                                            批内差:取同批次试剂盒对低、?#23567;?#39640;值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
                                            批间差:选取3个不同批次的试剂盒分别对低、?#23567;?#39640;值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
                                            批内差: CV<10%
                                            批间差: CV<12%

                                            线性

                                            在定值血清及血浆样本内加入适量的补体成分3a(C3a),并倍?#35748;?#37322;成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中补体成分3a(C3a)含量的测定值与理论值的比率。

                                            样本 1:2 1:4 1:8 1:16
                                            serum(n=5) 81-93% 95-105% 78-96% 95-105%
                                            EDTA plasma(n=5) 80-92% 92-102% 86-94% 86-103%
                                            heparin plasma(n=5) 97-104% 89-103% 79-101% 96-104%

                                            稳定性

                                            经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
                                            为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环?#31243;?#20214;需尽量保持一致,尤其是实验室内温?#21462;?#28287;度及温育条件。其次由同一实验员来进行操作可减少?#23435;?#35823;差。

                                            实验流程

                                            1. 实验前标准品、试剂及样本的准备;
                                            2. 加样(标准品及样本)100µL,37°C孵育1小时;
                                            3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
                                            4. 洗板3次;
                                            5. 加检测溶液B100µL,37°C孵育30分钟;
                                            6. 洗板5次;
                                            7. 加TMB底物90µL,37°C孵育10-20分钟;
                                            8. 加终止液50µL,立即450nm读数。

                                            实验原理

                                            将补体成分3a(C3a)抗体包被于96孔微?#35013;?#20013;,制成固相载体,向微孔中分别加入标准品或标本,其中的补体成分3a(C3a)与连接于固相载体?#31995;?#25239;体结合,然后加入生物素化的补体成分3a(C3a)抗体,将未结?#31995;?#29983;物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样?#20998;?#30340;补体成分3a(C3a)?#25910;?#30456;关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓?#21462;?

                                            相关产品

                                            编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
                                            RPA387Ra01 补体成分3a(C3a)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
                                            PAA387Ra01 补体成分3a(C3a)多克隆抗体 WB; IHC; ICC; IP.
                                            SEA387Ra 补体成分3a(C3a)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
                                            KSA387Ra01 补体成分3a(C3a)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

                                            参考文献

                                            杂志 参考文献
                                            22 Immobilization of soluble complement receptor 1 on islets. [ScienceDirect: S0142961211002596]
                                            Journal of Reproductive Immunology Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia [PubMed: 23415845]
                                            Journal of Antimicrobial Chemotherapy Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model [Pubmed: 23645587]
                                            SpringerPlus Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats [Pubmed: 24353977]
                                            Gynecol Obstet Invest Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? [PubMed: 26485247]
                                            Am J Reprod Immunol Human IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo Study [PubMed: 26388133]
                                            Journal of Neuroimmunology Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis [Pubmed:27235358]
                                            Plos One Alternative Pathway Inhibition by Exogenous Factor H Fails to Attenuate Inflammation and Vascular Leakage in Experimental Pneumococcal Sepsis in Mice [Pubmed:26872035]
                                            Digital Repository Dosagem de fra??es ativadas do sistema complemento em empiema induzido em ratos [10183]
                                            Colloids Surf B Biointerfaces. Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns. [pubmed:28131092]
                                            Pediatric Pulmonology Accuracy of complement activation product levels to detect infected pleural effusion in rats [pubmed:28474458]
                                            Journal of Immunology The Complement C3a–C3aR Axis Promotes Development of Thoracic Aortic Dissection via Regulation of MMP2 Expression [Pubmed:29367209]
                                            Artificial?Cells?Nanomedicine and Biotechnology Journal Long term safety and immunological effects of a nanobiotherapeutic, bovine poly-[hemoglobin-catalase-superoxide dismutase-carbonic anhydrase], after four weekly?… [Pubmed:29873524]
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